The Definitive Guide to cytometers
The Definitive Guide to cytometers
Blog Article
At the moment, the early signals have been dismissed as particles, but the retrospective analysis shown shared properties with the problematic cells in culture. Tracing the infection aided the lab set up additional laboratory processes for screening and security of assay-vital mobile lines.
As samples are obtained over the Attune CytPix Flow Cytometer, the higher-pace brightfield camera captures and shops photos of detected activities at a fee around six,000 pictures per 2nd, based on the flow fee and impression dimensions. For better overall flexibility, Attune Cytometric Computer software permits you to regulate the graphic capture frequency as required and choose a selected gate for graphic seize, if essential.
Incorporate acoustic concentrating technological know-how with high-parameter Examination for versatile spectral unmixing and conventional workflows.
Discover new insights into your experimental data by visualizing populations utilizing mixed fluorescence and morphology details. Our program interface wizards allow downsampling, concatenation (merged files) and dimensionality applications that happen to be built to the application for an intuitive, streamlined details Investigation encounter.
"An image is just not fairly worthy of 1000 dots, but it surely seriously really helps to see visuals. It is great to view pictures that correlate with staining. For example, AnnexinV-favourable cells which can be morphologically diverse than healthy cells, or CD14+ monocytes that happen to be greater and even more textured than CD3+/CD4+/CD14- lymphocytes.
Here, a highly trained user has gated singlets confidently. Immediately after analyzing the handbook singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate incorporates a lot more than 4% aggregates.
Optimizing gating approach. Even sturdy guide singlet gating is error-vulnerable and stays a subjective final decision point in Nearly all flow cytometry assays. Imaging can be employed to confirm and regulate gates to incorporate only single cells of curiosity.
Our experts will design and style panels for yourself as often times you would like—free of charge, no item order essential.
Early signs of this contamination had in the beginning been dismissed as debris. Backgating to find new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also allows you to use morphological functions to find out interesting subpopulations that would not be evident from flow cytometry details by yourself.
End users can look at function pictures in authentic-time to confirm sample high quality, enhance protocols, and history the morphology of every cell populace determined for Assessment. Imaging capture also Gains from acoustic focusing, which helps to situation the cells in order that a pointy, centered picture is obtained.
AI-driven automatic graphic Investigation With all the Attune Cytometric Computer software interprets event attributes into unique morphology parameters which can be coupled with conventional fluorescence and scatter parameters to reveal remarkably reproducible inhabitants variations and one of a kind subsets.
We Earlier shown the strength of imaging CAR-T/Ramos mobile interactions. Enable’s evaluate just the inhabitants of finest desire, the double good activities, To find out more. We are able to now use extended morphological parameters (in this article, circularity and skewness of depth) to even further examine the features of those populations and refine gating on these activities, rising facts robustness.
Leverage the strength of Innovative know-how and innovation to accelerate your discoveries. Belief from the Attune Flow Cytometer household to deliver the trustworthiness, velocity, and accuracy that set your do the job aside.
No wash/no lyse leukocyte Evaluation with imaging. Cells had been obtained from read more 24-hour-old blood by dilution in 1 mM EDTA ( Professional UX/UI permits buyers to train AI models for expanded purposes. (A) The “Coach Design” feature delivers annotation equipment and serious-time product teaching accuracy.
At the guts of every Attune Flow Cytometer lies our groundbreaking acoustic concentrating technologies, which utilizes audio waves to meticulously align cells throughout the flow cell. This progressive method surpasses standard hydrodynamic solutions, resulting in a lot quicker processing speeds, Excellent knowledge good quality, and greater sample throughput.
The Attune Xenith cytometer can be a higher-parameter spectral analyzer, delivering the two spectral unmixing and traditional flow capabilities for complete and adaptable cell Assessment.
The Attune CytPix Flow Cytometer lets consumers to visualize samples during acquisition, confirm the precision of gating strategies, and assist outcomes with trustworthy documentation for publication needs.
What gave the impression to be a homogeneous populace is in fact extra numerous—an Perception that needs to be regarded when interpreting success.