5 Tips about attune flow cytometer You Can Use Today
5 Tips about attune flow cytometer You Can Use Today
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Information-enriched characterization of mobile populations. Morphological data from photographs can incorporate on the richness of flow cytometry info. For instance, the figure reveals an in any other case standard apoptosis assay making use of Annexin V and PI, adding cell imaging to characterize cells in Each and every population to reveal morphologically unique attributes. These insights couldn't are gained from multiplex staining by itself.
As samples are obtained within the Attune CytPix Flow Cytometer, the higher-speed brightfield camera captures and retailers pictures of detected situations in a charge nearly 6,000 photographs per 2nd, with regards to the flow price and impression size. For higher versatility, Attune Cytometric Computer software permits you to regulate the impression seize frequency as required and choose a particular gate for impression capture, if essential.
(B) Uncooked 264.seven macrophages are often annotated inaccurately with regard to particle count because of their complexity and noncircular borders. The volume of singlets obtainable for Examination raises after the consumer trains and employs a far more accurate model.
The award-winning Invitrogen Attune CytPix Flow Cytometer combines acoustic concentrating by using a significant-speed brightfield digital camera for simultaneous superior throughput flow cytometry and superior resolution brightfield imaging.
"An image isn't really pretty really worth one thousand dots, nevertheless it actually really helps to see pictures. It is excellent to find out images that correlate with staining. One example is, AnnexinV-positive cells which can be morphologically different than healthy cells, or CD14+ monocytes which might be greater plus much more textured than CD3+/CD4+/CD14- lymphocytes.
Listed here, a seasoned consumer has gated singlets confidently. Just after assessing the guide singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate includes in excess of four% aggregates.
Pair your Attune flow cytometer by having an autosampler for increased workflow performance, and add robotic integration for optimum throughput.
Steady picture high quality even at high throughput Acoustic focusing and a significant-speed digicam Blend to graphic these T cells constantly at small or higher flow prices. Effortlessly alter emphasis and digicam settings to fulfill experimental needs.
Early signs of this contamination experienced at first been dismissed as particles. Backgating to find out new populations. Backgating imaged cells on the Attune CytPix Flow Cytometer also means that you can use morphological functions to find intriguing subpopulations that website might not be clear from flow cytometry information alone.
Probably most significantly, these occasions comprise cells of clearly distinct phenotypes which could lead to incorrect conclusions regarding double constructive functions (specifically in uncommon populations).
AI-driven automated picture Evaluation With all the Attune Cytometric Computer software translates event capabilities into unique morphology parameters that could be coupled with normal fluorescence and scatter parameters to expose hugely reproducible population adjustments and distinctive subsets.
Software Be aware: Automatic impression Investigation lessens consumer-to-person variability in flow cytometry gating strategies
Leverage the strength of State-of-the-art technologies and innovation to accelerate your discoveries. Rely on during the Attune Flow Cytometer family to provide the dependability, pace, and accuracy that set your get the job done aside.
No wash/no lyse leukocyte Examination with imaging. Cells had been obtained from 24-hour-outdated blood by dilution in 1 mM EDTA ( Pro UX/UI permits users to prepare AI styles for expanded applications. (A) The “Educate Design” element provides annotation instruments and actual-time design coaching precision.
At the center of every Attune Flow Cytometer lies our groundbreaking acoustic concentrating engineering, which utilizes sound waves to meticulously align cells in the flow cell. This impressive approach surpasses standard hydrodynamic solutions, resulting in more rapidly processing speeds, exceptional knowledge top quality, and enhanced sample throughput.
Because the cell line had previously been analyzed around the Attune CytPix Flow Cytometer, the scientists went back to the pictures and have been able to document the microbial infection at least 5 days earlier.
Agilent Value Promise ensures you a minimum of ten years of instrument use through the day you buy. If not, We're going to credit you Using the residual worth of the method toward an upgraded model.
What appeared to be a homogeneous population is definitely additional diverse—an insight that ought to be regarded as when interpreting outcomes.